Part:BBa_K1402004
AtFatA-CDS
Acyl-acyl carrier protein (ACP) thioesterase catalyzes the hydrolysis of acyl-ACP to fatty acids and ACP this reaction removes the coupling between fatty acid biosynthesis and phospholipid biosyntheis,which is a key to obtain free fatty acids. This thioesterase AtFatA can produce fatty acids with 16~18 carbon chain length. In previous researches, thioesterase gene AtFatA can be expressed in E.coli BL21(DE3) and enhance the synthesis of free fatty acids.This part can increase the productivity of free fatty acids. However, this part can not express itself. You should add promoter, RBS and terminator to it or use other parts we offer.
After the overexpression of AtFatA gene, we examined the species of fatty acid in the compound we got. There turned out to be 6 kinds of fatty acid, namely fatty acid of 14C chain length, 15C chain length with double-band, 16C chain length, 17C chain length with double-band, 18C chain length and 18C chain length with double-band. Among them, fatty acid of 16C chain length accounts for the greatest amount of 45.07% and fatty acid of 18C chain length is the least amount of 4.04%. Proportion of fatty acid of 14C, 15C with double-band, 17C with double-band and 18C with double-band is 5.61%, 7.96%, 16.21% and 21.11% respectively.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 365
Illegal BglII site found at 540
Illegal XhoI site found at 923 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1263
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